Curriculum Vitae

Cyril Dominguez

Born in August the 6th, 1975, French

Education

Ph.D in Chemistry in the NMR group of Utrecht University, The Netherlands (2000-2004)
Received degree in June the 14th, 2004

NMR-based docking of protein-protein complexes
Research promotors: Prof Rob Kaptein, Prof. Rolf Boelens
Copromotor: Dr. Alexandre Bonvin

Master degree in Nutritional Biochemistry, University of Aix-Marseille III, France (1999)
Research Director: Dr. Françoise Guerlesquin, BIP laboratory, CNRS

Oral presentations

Swiss RNA Workshop, Bern, Switzerland, 14 September 2007
"NMR studies of the complex between human hnRNP F and G-tract RNA: a novel mode of RNA recognition"

Euresco conference: NMR in molecular biology, Obernai, France, 5-10 September 2003
"HADDOCK: a protein-protein docking approach based on biochemical or biophysical data"

NWO protein annual meeting, Lunteren, the Netherlands, 9-10 December 2002
"HADDOCK: a protein-protein docking approach based on biochemical or biophysical data"

Poster presentation and conferences

XXIIth ICMRBS, Göttingen, Germany, 20-25 August 2006
"NMR studies of the complex between human hnRNP F and G-tract RNA: a novel mode of RNA recognition"
C. Dominguez, F. H-.T. Allain

NMR: A Tool For Biology VII, Institut Pasteur, Paris, France, 31 January - 1 February 2006

NMR: A Tool For Biology VI, Institut Pasteur, Paris, France, 2-4 February 2004

7th European NMR Large Scale Facilities User meeting, Oosterberk, 21-22 november 2003
“Structure of UbcH5B using a combination of homology modeling, NMR relaxation and NOE data”
K. Houben, C. Dominguez, F.M.A. van Schaik, H.Th.M. timmers, A.M.J.J. Bonvin, R. Boelens
“Insight into the E2-E3 specificity in the ubiquitination pathway from the UbcH5B/CNOT4 complex”
C. Dominguez, A.M.J.J. Bonvin, G.S. Winkler, F.M.A. van Schaik, H.Th.M. Timmers, R. Boelens
“HADDOCK (High Ambiguity Driven DOCKing): a protein-protein docking approach based on biochemical and/or biophysical data”
C. Dominguez, A.D.J. van Dijk, R. Boelens, A.M.J.J. Bonvin

ICMRBS, Toronto, Canada, 25-30 August 2002
“HADDOCK (High Ambiguity Driven DOCKing): a protein-protein docking approach based on biochemical and/or biophysical data”
C. Dominguez, R. Boelens, and A.M.J.J. Bonvin.
“Studies of the PB1-PC domain complex combining NMR and docking”
A. Petit, C. Dominguez, A.M.J.J. Bonvin, C. Zwahlen, and M. Peter.

NMR: A Tool For Biology V, Institut Pasteur, Paris, France, 4-6 February 2002

5th European NMR Large Scale Facilities User meeting, Frankfurt, Germany, 8-11 November 2001
“A NMR study on the interaction of the human transcriptional factor Not4 Ring finger and an ubiquitin-conjugating enzyme, UbcH5B”
C. Dominguez, H. Hanzawa, T.K. Albert, M.J. de Rouwe, F.M.A. van Schaik, H.T.M. Timmers, R. Boelens

Courses and workshops

2003: - Eurolab course, Florence Italy, 31 August – 5 september
Advanced computing in NMR spectroscopy
Instructor for the practical “NMR constraints for solution structure determination” together with Pr. Dr. R. Boelens

2003: - Solid-state NMR: Basic principles and biophysical applications
Marc Baldus, Utrecht, the Netherlands

2001: - EMBO Course, Heidelberg, Germany, 12-19 September
Structure determination of biological macromolecules by solution NMR

2000: - EMBO Course, Il Ciocco, Lucca, Italy, 13-18 August
Multidimensional NMR in structural biology

Experimental experiences

- NMR:
Data acquisition (BRUKER)
Data processing (XWINNMR, NMRPipe)
Spectra analysis (NMRView, Sparky)
Structure calculation and refinement (CNS, Aria, AtnosCandid, Amber)
Modeling (SwissModel, Modeller)
Docking (BIGGER, HADDOCK)

- PROTEIN ENGINEERING:
Cloning techniques
Site-directed mutagenesis techniques
Expression of recombinant proteins using heterologous systems (E.
coli)
Isotope labeling
Protein purification
Protein analysis (SDS-PAGE)
In vitro RNA transcription
RNA purification