Curriculum Vitae

Antoine CLERY, Dr

Date of birth: June the 8th, 1977
Nationality: French
Married, 2 children

ACADEMIC BACKGROUND:

Oberassistent in Structural Biology (2011)
ETH Zurich, Switzerland
Frederic Allain's lab

Post doc in Structural Biology (2007)
ETH Zurich, Switzerland
Frederic Allain's lab

Ph. D. in Molecular Biology with honors (2006)
University of Nancy I, France
Christiane Branlant's lab
Ph. D. project: Structural and functional study of “K-turn” RNA motifs in the assemblage of ribonucleoproteique particles involved in the RNAs maturation.

Master degree in Microbiology, Enzymology, Nutrition with honors, first position (2001)
University of Nancy I, France

TEACHING: (360 hours)
From October 2001 to August 2006: Teaching courses in molecular biology to undergraduate students, demonstrations of genetic engineering to postgraduate students.
2001-2003: Monitorat
2004-2006: 1/2 ATER

FELLOWSHIP:
EMBO fellowship (01/2008 to 12/2009)

Associate F1000 Member (since 2010)
http://f1000.com/thefaculty/member/4882433725057756

EXPERIMENTAL SKILLS

1. In molecular biology
• Protein and RNA engineering
Cloning, site-directed mutagenesis
Expression of recombinant proteins in E. coli and purification
Transcription of RNA in vitro and purification and experimental determination of its secondary structure in vitro by chemical and enzymatic probing

• Protein-RNA interactions
In vitro:
Localization of a protein binding site on RNA by chemical and enzymatic footprinting
Determination of affinity between protein and RNA by ITC measurement and gel shift assays
Determination of the RNA sequence having the strongest affinity for a protein by SELEX experiment
In vivo:
Northern blot and western blot to determine the level of RNA and protein expression in vivo
Immunoprecipitations using tagged proteins to test their interaction with RNA in vivo
In vivo splicing assays

2. In structural biology (NMR)
Production of labeled protein and RNA in large scale for NMR study
NMR titrations to quickly determine the binding interface of a RNA-protein complex
Structure determination of protein free and bound to RNA
Dynamic studies to evaluate the flexibility of protein backbone in the free and RNA-bound state

ORAL PRESENTATIONS

CLERY A., GOURRIER N., SEGAULT V., CHARPENTIER B., LECLERC F., FOURNIER R. and BRANLANT C.
Etude structurale, fonctionnelle et phylogénétique des sites de reconnaissance de la protéine Snu13/15,5 kDa sur le snoRNA U3
Sifr ARN Meeting, Nancy (France), 14-17 octobre 2002

CLERY A., DOMINGUEZ C. and ALLAIN F.H.-T
NMR structure of the tra2beta1 splicing factor in complex with RNA revealed an unexpected extensive interaction with the C-terminal extremity of the RRM
Swiss RNA meeting, Bern (Switzerland), January 30th, 2009

CLERY A., DOMINGUEZ C. and ALLAIN F.H.-T
NMR structural study of human tra2beta1 and SF2/ASF, two proteins involved in the alternative splicing of SMN
Swiss National Science Foundation (NCCR), Zürich (Switzerland), March 23th, 2009

CLERY A., DOMINGUEZ C. and ALLAIN F.H.-T
NMR structure of human tra2beta1, a protein involved in the alternative splicing of SMN
Alternative and aberrant splicing in neuromuscular and neurodegenerative processes (EURASNET*), Trieste (Italy), March 26-27th, 2009

CLERY A., BENDERSKA N., HAUTBERGUE G.M., DOMINGUEZ C., WILSON S.A., STAMM S. and ALLAIN F.H.-T *
Two very different recognition modes of purine-rich RNA sequence by the human RS-rich splicing regulators Tra2beta1 and ASF/SF2
Cold Spring Harbor meeting (eukaryotic mRNA processing), New York (US), August 18-22th, 2009

* The presented structures were published on the front cover of the abstract book.

CLERY A., BENDERSKA N., JAYNE S., DOMINGUEZ C., STAMM S. and ALLAIN F.H.-T
Molecular basis of the SMN exon7 recognition by the human SR-like protein Tra2beta1
RNA mis-splicing: disease and therapeutics (EURASNET*), Cambridge (UK), July 12-14, 2010

*EURASNET: European Alternative Splicing Network of Excellence

CLERY A., SINHA R., MOURSY A., DAUBNER G., KRAINER A. and ALLAIN F.H.-T
The structure of SRSF1 pseudo-RRM in complex with RNA questions the dogma of RRM-RNA interaction
Protein kinases regulating RNA splicing, Roscoff (France), November 12-16, 2011

CLÉRY A.*, SINHA R., CORRIONERO A., MOURSY A., DAUBNER G., VALCÁRCEL J., KRAINER A.R. and ALLAIN F.H.-T
Sequence-specific RNA recognition by a conserved alpha-helix of the pseudo-RRM of SR proteins regulates pre-mRNA splicing
Recent Trends in NMR of biomolecules and Advanced Materials, Pune (India), January 23-25, 2012

* invited speaker

CLÉRY A., SINHA R., CORRIONERO A., MOURSY A., DAUBNER G., VALCÁRCEL J., KRAINER A.R. and ALLAIN F.H.-T
Sequence-specific RNA recognition by a conserved alpha-helix of the pseudo-RRM of SR proteins regulates pre-mRNA splicing
Swiss RNA meeting, Bern (Switzerland), February 3rd, 2012

CLÉRY A., SINHA R., CORRIONERO A., MOURSY A., DAUBNER G., VALCÁRCEL J., KRAINER A.R. and ALLAIN F.H.-T
Sequence-specific RNA recognition by a conserved alpha-helix of the pseudo-RRM of SR proteins regulates pre-mRNA splicing
Eurasnet meting, Trieste (Italy), March 24-28, 2012

POSTER PRESENTATIONS

CLERY A., SEGAULT V., CHARPENTIER B., GOURRIER N., LECLERC F. and BRANLANT C.
Etude comparative de l’interaction des motifs ARN sélectionnés par la technique SELEX avec les protéines Snu13 de S. cerevisiae et son homologue L7Ae chez P. abyssi
Sifr ARN Meeting, Nancy (France), October 14-17, 2002

CLERY A., MARMIER-GOURRIER N., SENTY-SÉGAULT V., CHARPENTIER B., SCHLOTTER F., LECLERC F. and BRANLANT C.
A structural, phylogenetic and functional study of 15.5kDa/Snu13p protein binding on U3 small nucleolar RNA
6th International Conference on Ribosome Synthesis, Palais des Congrès, Arcachon (France), June 6-10, 2003

CLERY A., SENTY-SEGAULT V., MARMIER-GOURRIER N., SCHLOTTER F. and BRANLANT C.
Structural and Functional study on Saccharomyces cerevisiae U3 snoRNP
19th International Workshop on the Cell Nucleus (Wilhelm Bernhard workshop), Münsterschwarzach Abbey (Germany), September 1-5, 2005

CLERY A., DOMINGUEZ C., and ALLAIN F.H.-T
NMR study of the tra2beta1 splicing factor in interaction with RNA
13th Annual Meeting of the RNA Society, Berlin (Germany), July 28-August 3, 2008

CLERY A., DOMINGUEZ C., and ALLAIN F.H.-T
NMR structure of the tra2beta1 splicing factor in complex with RNA revealed an unexpected extensive interaction with the C-terminal extremity of the RRM
Advances in NMR of protein and nucleic acid molecular recognition, Murnau (Germany), Oct 16-18, 2008

CLERY A., BENDERSKA N., HAUTBERGUE G.M., DOMINGUEZ C., WILSON S.A., STAMM S. and ALLAIN F.H.-T
Two very different recognition modes of purine-rich RNA sequence by the human RS-rich splicing regulators Tra2beta1 and ASF/SF2
7th international NCCR symposium in structural biology, Zurich (Switzerland), September 7-8th, 2009

CLERY A., BENDERSKA N., JAYNE S., DOMINGUEZ C., STAMM S. and ALLAIN F.H.-T
Molecular basis of purine-rich recognition by the SR-like protein Tra2beta1
EURASNET Annual Reporting Meeting, Lisboa (Portugal), March 1-4, 2010

CLERY A., BENDERSKA N., JAYNE S., DOMINGUEZ C., STAMM S. and ALLAIN F.H.-T
Molecular basis of purine-rich recognition by the SR-like protein Tra2beta1
15th Annual Meeting of the RNA Society, Seattle (US), June 22-26, 2010

CLERY A., JAYNE S., BENDERSKA N., DOMINGUEZ C., STAMM S. and ALLAIN F.H.-T
Molecular basis of the SMN exon7 recognition by the human SR-like protein Tra2beta1
8th international NCCR symposium in structural biology, Zurich (Switzerland), September 2-3th, 2010

CLERY A., JAYNE S., BENDERSKA N., DOMINGUEZ C., STAMM S. and ALLAIN F.H-T
Molecular basis of the SMN exon 7 recognition by the human SR-like protein Tra2-beta1
Second International EURASNET Conference on Alternative Splicing, Granada (Spain), from 28 February to 3 March 2011

CLERY A., SINHA R., MOOS D., MOURSY A., DAUBNER G., KRAINER A. and ALLAIN F.H.-T
The structure of SRSF1 pseudo-RRM in complex with RNA revealed an unexpected mode of recognition
8th European Federation of EPR Groups Meeting (EUROMAR), Frankfurt am Main (Germany), August 21st -25th, 2011

CLERY A., SINHA R., MOOS D., MOURSY A., DAUBNER G., KRAINER A. and ALLAIN F.H.-T
The structure of SRSF1 pseudo-RRM in complex with RNA revealed an unexpected mode of recognition
9th international NCCR symposium in structural biology, Zurich (Switzerland), September 1-2, 2011