Cardiotoxins from snake venoms produce a variety of toxic
effects, e.g. depolarization of membranes, hemolysis and
synergistic action with phospholipase A2. Relatively little is
known about the mechanisms of action of cardiotoxins and
additional insight might come from a knowledge of the spatial
structures. We present work on the determination of the spatial
structure of Cardiotoxin VII2 from N.moss.moss., following a
strategy based on the use of 2-dimensional 1H NMR at 500 MHz as
was recently described in detail (K. Wüthrich, G. Wider, G.
Wagner and W. Braun, J. Mol. Biol. 155, 311-319 (1982)). Nearly
complete 1H NMR assignments will be described and the gross
features of the conformation of toxin VII2 will be diuscussed.